Subtyping animal influenza virus with general multiplex RT-PCR and Liquichip high throughput (GMPLex)

Molecular detection and subtyping of human influenza A viruses based on multiplex RT-PCR assay

Influenza virus infections have been causing major public health concerns worldwide. Therefore, rapid and accurate diagnostic methods for typing and subtyping of influenza virus are crucial both for patient management and to limit dissemination. In this study, multiplex RT-PCR was developed, optimized and evaluated for detection, typing and subtyping of influenza viruses. The typing assay consi...

Multiplex Reverse Transcriptase-PCR Assay for Typing and Subtyping of Influenza A (H5 & H9) Virus in Iran

Avian influenza virus (AIV) infection is a major cause of bird or human mortality and morbidity, therefore the rapid identification of the virus is of important clinical and epidemiological implication. Methods: A multiplex Reverse Transcriptase PCR (RT-PCR) was optimized for the detection of influenza A virus and the H5 and H9 subtypes. The influenza type A specific primers were directed to t...

Typing and subtyping influenza virus using DNA microarrays and multiplex reverse transcriptase PCR.

A model DNA microarray has been prepared and shown to facilitate typing and subtyping of human influenza A and B viruses. Reverse transcriptase PCR was used to prepare cDNAs encoding approximately 500-bp influenza virus gene fragments, which were then cloned, sequenced, reamplified, and spotted to form a glass-bound microarray. These target DNAs included multiple fragments of the hemagglutinin,...

Development of Real Time RT-PCR Assays for Neuraminidase Subtyping of Avian Influenza Virus

Rapid detection and serotyping of dengue virus by multiplex RT-PCR and real-time SYBR green RT-PCR.

INTRODUCTION Dengue fever and dengue haemorrhagic fever currently rank highly among the newly-emerging infectious diseases, and are considered to be the most important arboviral disease worldwide. The definitive diagnosis is culture analysis, but practical considerations limit its use. Also, the period for viral detection is limited. Within a day or two after fever subsides, rising levels of an...